TLC Analysis of Analgesic Drugs - 1291 Words

January 24, 2013 TLC Analysis of Analgesic Drugs Introduction: The purpose of this lab was to determine the composition of four different over-the-counter drugs (Anacin, Bufferin, Excedrin, and Tylenol). These compositions were determined by using the method of thin-layer chromatography (TLC) of the four over-the-counter drugs, which were then compared to four different components of drugs (acetaminophen, aspirin, caffeine, and salicylamide). Experiment Scheme1: Figure 1. Structure formulas1 Under an open flame in the hood, 12 capillary micropipettes were made. Two silica TLC plates were obtained, along with a development chamber. Each plate was prepared and labeled appropriately with a lead pencil.†¦show more content†¦Without the mark, it was hard to differentiate between aspirin and salicylamide when comparing it to the reference spot on the same page, and in comparing it to the sample plate. It was unclear to determine which of the two components were present in Bufferin. Next time, the spots will be marked appropriately as stated and those that fluoresce will receive an â€Å"X†2. Another error that was encountered was mishandling of the plates. It was stated to handle the plates with care and to avoid touching the top because residue would affect the experiment. The oils that are on the body could potentially throw off the result, which in turn leads to misleading data to interpret. It wasn’t until the plate (reference) was placed within the iodine chamber that a fingerprint became noticeable closer to the top. Although it may or may not have caused any significant skews in the data, it was a precaution that was overlooked. Next time, tweezers should be used more often and in the off chance that the hands need to hold the plate, it would be important handle from the edges instead of a big portion on the top. 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